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从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用 Article
Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee
《工程(英文)》 2024年 第32卷 第1期 页码 42-57 doi: 10.1016/j.eng.2023.08.001
A universal vaccine is in high demand to address the uncertainties of antigenic drift and the reduced effectiveness of current influenza vaccines. In this study, a strategy called computationally optimized broadly reactive antigen (COBRA) was used to generate a consensus sequence of the hemagglutinin globular head portion (HA1) of influenza virus samples collected from 1918 to 2021 to trace evolutionary changes and incorporate them into the designed constructs. Constructs carrying different HA1 regions were delivered into eukaryotic cells by Salmonella-mediated bactofection using a Semliki Forest virus RdRp-dependent eukaryotic expression system, pJHL204. Recombinant protein expression was confirmed by Western blot and immunofluorescence assays. Mice immunized with the designed constructs produced a humoral response, with a significant increase in immunoglobulin G (IgG) levels, and a cell-mediated immune response, including a 1.5-fold increase in CD4+ and CD8+ T cells. Specifically, constructs #1 and #5 increased the production of interferon-γ (IFN-γ) producing CD4+ and CD8+ T cells, skewing the response toward the T helper type 1 cell (Th1) pathway. Additionally, interleukin-4 (IL-4)-producing T cells were upregulated 4-fold. Protective efficacy was demonstrated, with up to 4-fold higher production of neutralizing antibodies and a hemagglutination inhibition titer > 40 against the selected viral strains. The designed constructs conferred a broadly protective immune response, resulting in a notable reduction in viral titer and minimal inflammation in the lungs of mice challenged with the influenza A/PR8/34, A/Brisbane/59/2007, A/California/07/2009, KBPV VR-92, and NCCP 43021 strains. This discovery revolutionizes influenza vaccine design and delivery; Salmonella-mediated COBRA-HA1 is a highly effective in vivo antigen presentation strategy. This approach can effectively combat seasonal H1N1 influenza strains and potential pandemic outbreaks.
关键词: COBRA Influenza A Salmonella Vaccine Broad spectral protection
Applying the spectral stochastic finite element method in multiple-random field RC structures
Abbas YAZDANI
《结构与土木工程前沿(英文)》 2022年 第16卷 第4期 页码 434-447 doi: 10.1007/s11709-022-0820-6
关键词: uncertainty spectral stochastic finite element method correlation length reliability assessment reinforced concrete beam/slab
Yun KONG, Tianyang WANG, Zheng LI, Fulei CHU
《机械工程前沿(英文)》 2017年 第12卷 第3期 页码 406-419 doi: 10.1007/s11465-017-0419-0
Planetary transmission plays a vital role in wind turbine drivetrains, and its fault diagnosis has been an important and challenging issue. Owing to the complicated and coupled vibration source, time-variant vibration transfer path, and heavy background noise masking effect, the vibration signal of planet gear in wind turbine gearboxes exhibits several unique characteristics: Complex frequency components, low signal-to-noise ratio, and weak fault feature. In this sense, the periodic impulsive components induced by a localized defect are hard to extract, and the fault detection of planet gear in wind turbines remains to be a challenging research work. Aiming to extract the fault feature of planet gear effectively, we propose a novel feature extraction method based on spectral kurtosis and time wavelet energy spectrum (SK-TWES) in the paper. Firstly, the spectral kurtosis (SK) and kurtogram of raw vibration signals are computed and exploited to select the optimal filtering parameter for the subsequent band-pass filtering. Then, the band-pass filtering is applied to extrude periodic transient impulses using the optimal frequency band in which the corresponding SK value is maximal. Finally, the time wavelet energy spectrum analysis is performed on the filtered signal, selecting Morlet wavelet as the mother wavelet which possesses a high similarity to the impulsive components. The experimental signals collected from the wind turbine gearbox test rig demonstrate that the proposed method is effective at the feature extraction and fault diagnosis for the planet gear with a localized defect.
关键词: wind turbine planet gear fault feature extraction spectral kurtosis time wavelet energy spectrum
Xiaoming Yuan,Jiahui Chen,Ning Zhang,Qiang Ye,Changle Li,Chunsheng Zhu,Xuemin Sherman Shen,
《工程(英文)》 doi: 10.1016/j.eng.2023.04.015
关键词: Knowledge sharing Internet of Vehicles Federated learning Broad learning Reconfigurable intelligent surfaces Resource allocation
Fu-qing Liang,Yong-ping Sun
《工程管理前沿(英文)》 2014年 第1卷 第4期 页码 330-335 doi: 10.15302/J-FEM-2014055
关键词: environmental science the Three Gorges cultural relics innovate environmental protection measures green protection
Fei Zhang,Minghao Liao,Mingbo Pu,Yinghui Guo,Lianwei Chen,Xiong Li,Qiong He,Tongtong Kang,Xiaoliang Ma,Yuan Ke,Xiangang Luo,
《工程(英文)》 doi: 10.1016/j.eng.2023.07.008
Huaqiang CHU, Qiang CHENG, Huaichun ZHOU, Fengshan LIU
《能源前沿(英文)》 2011年 第5卷 第2期 页码 149-158 doi: 10.1007/s11708-010-0124-8
Application of spectral technology in flame measurement
Jiaxun LIU, Xiaoshu CAI, Zenghao ZHU, Huinan YANG
《能源前沿(英文)》 2014年 第8卷 第1期 页码 138-143 doi: 10.1007/s11708-013-0283-5
关键词: spectrum emissivity measurement component of fuel near-infrared spectrometer
ZHANG Liquan, GAO Zhanguo, Armitage Richard, Kent Martin
《环境科学与工程前沿(英文)》 2008年 第2卷 第2期 页码 187-197 doi: 10.1007/s11783-008-0004-1
关键词: Spectral multi-seasonal hyperspectral invasive JR re-analysed
Zhigang GUO, Zhi SUN
《结构与土木工程前沿(英文)》 2011年 第5卷 第4期 页码 458-464 doi: 10.1007/s11709-011-0133-7
关键词: PZT piezoelectric impedance optimization spectral element damage identification
Rapid and long-effective removal of broad-spectrum pollutants from aqueous system by ZVI/oxidants
Sana Ullah, Xuejun Guo, Xiaoyan Luo, Xiangyuan Zhang, Siwen Leng, Na Ma, Palwasha Faiz
《环境科学与工程前沿(英文)》 2020年 第14卷 第5期 doi: 10.1007/s11783-020-1268-3
关键词: Zero-Valent Iron (ZVI) Oxidants Heavy Metals (HMs) Metalloids Nitrate Phosphate
李俊岳,杨运强,李桓,张晓囡,杨立军
《中国工程科学》 2001年 第3卷 第7期 页码 21-29
焊接电弧中含有丰富的光谱信息,利用这些信息对焊接电弧这一复杂的物理现象进行测试(或称诊断)和控制,是提高和发展焊接技术的一种非常有效的方法,具有重要的理论和实际意义。文章论述了这方面 研究的进展,阐明了电弧光谱信息的测控原理,介绍了电弧光谱信息在电弧诊断,电弧气氛监控,弧焊区成像,特别是近年来在脉冲MIG焊熔滴过渡控制等方面的应用情况。同时,还介绍了这一技术在等离子体现象中的一些重要应用,并总结出几点基本看法,指出了它的发展方向。
一种基于锚点的谱聚类方法 None
Qin ZHANG, Guo-qiang ZHONG, Jun-yu DONG
《信息与电子工程前沿(英文)》 2018年 第19卷 第11期 页码 1385-1396 doi: 10.1631/FITEE.1700262
关键词: 聚类;谱聚类;图拉普拉斯;锚点
CROP PROTECTION OPENS UP NEW ERA OF CONSERVATION AND UTILIZATION OF GREEN OPTIONS
《农业科学与工程前沿(英文)》 2022年 第9卷 第1期 页码 1-3 doi: 10.15302/J-FASE-2021431
China is the largest agricultural producer in the world. Reducing yield losses caused by pests is an important issue and major challenge for China, especially when confronting global climate change, biological invasions and declining agricultural biodiversity of recent decades. Wang et al. (this issue) summarized the impacts of changing climate on two staple crops in China, wheat and rice (https://doi.org/FASE-2021432). They reviewed the impacts of climate change on crops, crop pests and crop diseases. Basically, increased temperature would reduce crop yields and increase pest damage. Biological invasions have become a serious threat to the agriculture worldwide. Developing approaches for monitoring and controlling invasive pests, such as fall armyworm, has been recognized as high priority for China. The excessive use of highly toxic pesticides has caused considerable damage to the environment and biodiversity, which is also one of the many contributors to the increasing outbreaks of pests and diseases. In addition, understanding interaction between crop pests and plant diseases is another critical challenge for agriculture. About 80% plant viruses can be transmitted by crop pest vectors such as aphids, whiteflies and other hemipteran pests. Therefore, to solve the problem of crop pests and diseases, suitable green crop protection methods have been identified as a key components of sustainable development. This special issue of Frontiers of Agricultural Science and Engineering outlines potential conservation and utilization of green options for crop protection.
To prevent outbreaks of crop pests, an advanced monitoring and early warning system could be the first step in crop protection. The use of science and technology in monitoring and early warning of major crop pests provides better pest management and acts as a fundamental part of an integrated plant protection strategy to achieve the goal of sustainable development of agriculture. Wu et al. (this issue) summarized the fundamental information on pest monitoring and early warning in China by documenting the history of research and application, Chinese laws and regulations related to plant protection, and the National Monitoring and Early Warning System, with the purpose of presenting the Chinese model as an example of how to promote regional management of crop pests, especially cross border pests such as fall armyworm and locust, by international cooperation across pest-impacted countries (https://doi.org/10.15302/J-FASE-2021411). They provide an overview of long-term Chinese efforts in the fight against major pests and to present the country’s experience in crop pest monitoring and early warning technology to the world.
Field management is a traditional conservation agriculture technique on plant protection such as no-till cropping, crop rotation, intercropping and cover cropping. Shi et al. (this issue) found that soil solarization is an environmental-friendly promising strategy that achieved complete mortality to the larvae of Bradysia cellarum (https://doi.org/10.15302/J-FASE-2021402). Crop rotation, intercropping and cover cropping of different crops or plants could help to reduce the outbreak of crop pests and diseases. Wang et al. (this issue) summarized the interactions and trade-offs between plants and entomophagous arthropods, and discussed a landscape management method by sowing flowering plants in agricultural fields (https://doi.org/10.15302/J-FASE-2021427). However, such methods are limited when crop pests have already become well established in crop fields.
Pesticides are widely used in the crop protection. The ideal pesticide should have high effectiveness in the control crop pests but low toxicity to non-target organisms. In this case, biopesticides including biochemical pesticides, microbial pesticides and plant-incorporated protectants are considered to be potential ideal pesticides for crop protection. Biochemical pesticides are the naturally occurring substances, they are derived from animals, plants and microorganisms. Microbial pesticides mainly rely on bacteria, fungi, virus or protozoans as the active agent. They are environment-friendly biopesticides with high effectiveness and species-specificity, and have been commercialized worldwide, for example, Bacillus thuringiensis. Plant-incorporated protectants are products that are genetically incorporated into plant tissues including chemicals, proteins or dsRNA for pest control or anti-microbial activity. Qu et al. (this issue) systematically introduced bioinsecticides for pest control and discussed current constraints that prevent bioinsecticides from being widely used and proposed the future research directions in this issue (https://doi.org/10.15302/J-FASE-2021404). Xu et al. (this issue) optimized synthesis process of an anti-plant-virus candidate drug NK0238, and evaluated the antivirus activity and environmental safety of this product (https://doi.org/10.15302/J-FASE-2021390). Zhu et al. (this issue) investigated a new family of natural cyclic lipodepsipeptides with potent activity against Gram-positive bacteria. By genetic manipulation of accessory genes in the WAP biosynthetic gene cluster, new WAP-8294As were produced in Lysobacter, which confirmed the possibility to expand the spectrum of the biocontrol compounds (https://doi.org/10.15302/J-FASE-2021410). In addition, structural biology studies on action targets provides important insights on molecular functions. Using a structure-based drug design strategy, Zheng et al. (this issue) prepared a battery of novel triketone-quinoxaline compounds, which could be potentially applied to design new herbicides targeting p-hydroxyphenylpyruvate dioxygenase (https://doi.org/10.15302/J-FASE-2021401).
With the development and application of new biotechnology in agriculture, opportunities for crop protection are great becoming more abundant, opening new approaches, such as genetic engineering, to improve the plant resistance against crop pests and diseases. It is now feasible to control pests by directly targeting genes that are essential for the development and survival of insects and pathogenic microorganisms, for example, the sterile insect technique based on gene-drive and RNA interference using dsRNA. In addition, it has been confirmed that some gene families are effective targets that indirectly contribute to crop pests and diseases protection by targeting the plant biochemical synthesis or virus transmitting pathways. In this case, identifying new genes associated with the interaction between crops, insects, microorganisms should be a key focus, both now and in the future. Huang et al. (this issue) summarized the recent research on the interaction between plant viruses and insect vectors, and discussed the potential control strategies to prevent the transmission of insect-vectored plant viruses using RNAi technology, gene editing technology and gene-driven technology (https://doi.org/10.15302/J-FASE-2021389).
We anticipate that the ideas and approaches highlighted in this issue will help broaden reader’s perspectives on every increasing prospect for a new era of sustainable and environment-friendly agricultural pest management.
Bioengineering protection mechanism of city rock slope and its laboratory test
Desong JIANG, Minghua ZHAO, Chong JIANG,
《结构与土木工程前沿(英文)》 2009年 第3卷 第4期 页码 414-421 doi: 10.1007/s11709-009-0055-9
关键词: indoor engineering project protection mechanism different runoff lagging
标题 作者 时间 类型 操作
从H1N1血凝素序列提取的沙门氏菌传递的COBRA-HA1抗原对甲型流感亚型产生广谱保护作用
Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee
期刊论文
Applying the spectral stochastic finite element method in multiple-random field RC structures
Abbas YAZDANI
期刊论文
Fault feature extraction of planet gear in wind turbine gearbox based on spectral kurtosis and time wavelet
Yun KONG, Tianyang WANG, Zheng LI, Fulei CHU
期刊论文
Low-Cost Federated Broad Learning for Privacy-Preserved Knowledge Sharing in the RIS-Aided Internet of
Xiaoming Yuan,Jiahui Chen,Ning Zhang,Qiang Ye,Changle Li,Chunsheng Zhu,Xuemin Sherman Shen,
期刊论文
Implement and Innovate Environmental Protection Measures, Promote Green Protection of Cultural Relics
Fu-qing Liang,Yong-ping Sun
期刊论文
A Miniature Meta-Optical System for Reconfigurable Wide-Angle Imaging and Polarization-Spectral Detection
Fei Zhang,Minghao Liao,Mingbo Pu,Yinghui Guo,Lianwei Chen,Xiong Li,Qiong He,Tongtong Kang,Xiaoliang Ma,Yuan Ke,Xiangang Luo,
期刊论文
Nongray radiation from gas and soot mixtures in planar plates based on statistical narrow-band spectral
Huaqiang CHU, Qiang CHENG, Huaichun ZHOU, Fengshan LIU
期刊论文
Application of spectral technology in flame measurement
Jiaxun LIU, Xiaoshu CAI, Zenghao ZHU, Huinan YANG
期刊论文
Spectral characteristics of plant communities from salt marshes: A case study from Chongming Dongtan,
ZHANG Liquan, GAO Zhanguo, Armitage Richard, Kent Martin
期刊论文
Spectral element modeling based structure piezoelectric impedance computation and damage identification
Zhigang GUO, Zhi SUN
期刊论文
Rapid and long-effective removal of broad-spectrum pollutants from aqueous system by ZVI/oxidants
Sana Ullah, Xuejun Guo, Xiaoyan Luo, Xiangyuan Zhang, Siwen Leng, Na Ma, Palwasha Faiz
期刊论文